Long Noncoding RNA SNHG4 Attenuates the Injury of Myocardial Infarction via Regulating miR-148b-3p/DUSP1 Axis

Figure 3

Silencing of miR-148b-3p promotes the viability and represses the apoptosis and inflammation of hypoxia-induced H9c2 cells. (a) The expression of miR-148b-3p in hypoxia-induced H9c2 cells was detected by qRT-PCR. (b) The expression of miR-148b-3p in hypoxia-induced H9c2 cells transfected with miR-148b-3p inhibitor/inhibitor-NC was detected by qRT-PCR. (c) Cell viability (OD450) was measured by MTT assay. (d) Caspase-3 activity. (e) The protein expression of Bax and Bcl-2 were measured by western blot. (f–h) The levels of TNF-α, IL-6, and IL-1β were measured by ELISA. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)