Long Noncoding RNA SNHG4 Attenuates the Injury of Myocardial Infarction via Regulating miR-148b-3p/DUSP1 Axis

Figure 4

The target relationship between DUSP1 and miR-148b-3p. (a) A binding site of DUSP1 and miR-148b-3p was predicted by TargetScan and StarBase. (b) DLR assay of the target relationship between DUSP1 and miR-148b-3p. (c) The expression of miR-148b-3p in H9c2 cells transfected with miR-148b-3p mimics/miR-NC was detected by qRT-PCR. (d) The protein expression of DUSP1 in H9c2 cells transfected with miR-148b-3p inhibitor/inhibitor NC or miR-148b-3p mimics/miR-NC was detected by western blot. (e) The mRNA expression of DUSP1 in hypoxia-induced H9c2 cells was detected by qRT-PCR. (f) The protein expression of DUSP1 in hypoxia-induced H9c2 cells was detected by western blot. (g) The mRNA expression of DUSP1 in hypoxia-induced H9c2 cells transfected with sh-DUSP1/sh-NC/pcDNA-NC/pcDNA-DUSP1 was detected by qRT-PCR. (h) Cell apoptosis was detected by flow cytometry. .

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