Curcumin Attenuates Ferroptosis-Induced Myocardial Injury in Diabetic Cardiomyopathy through the Nrf2 Pathway

Figure 2

Curcumin improves myocardial cell damage. Nor-G: 5.5 mmol/L glucose concentration; H-G: 30 mmol/L glucose concentration; H-Cur-G: 30 mmol/L glucose concentration + curcumin. (a) CCK-8 assay detected myocardial cell activity at different curcumin concentrations. (b) Expression level and localization of Nrf2 assessed by immunofluorescence (×200). Fluorescence colocalization analysis showed that curcumin induced Nrf2 nucleus localization with a Pearson correlation coefficient of 0.59, indicating a strong correlation. (c) Apoptosis and intracellular ROS levels were measured using TUNEL and ROS fluorescent probes (×200). (d) Gpx4 cell expression was detected using immunofluorescence (×200). (e) The expression levels of Nrf2 protein in the nucleus and ferroptosis pathway-related proteins were detected by western blot ().

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