Curcumin Attenuates Ferroptosis-Induced Myocardial Injury in Diabetic Cardiomyopathy through the Nrf2 Pathway

Figure 3

High glucose-induced cardiomyocyte damage can be alleviated by overexpression of Nrf2. H-G: 30 mmol/L glucose concentration; WT-Nrf2-G: Nrf2 overexpressing+30 mmol/L glucose concentration; KD-Nrf2-G: knockdown of Nrf2 expression+30 mmol/L glucose concentration. (a) Virus transfection efficiency was determined using fluorescence, and the optimal multiplicity of infection value was 20 (×200). Western blot confirmed that Nrf2 was overexpressed and successfully knocked out among the experimental groups (). (b) The apoptosis rate of cardiomyocytes in each group was detected using terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) fluorescent probe. Overexpression of Nrf2 significantly alleviated myocardial death induced by high glucose concentrations (×200). (c) Immunofluorescence was used to detect the level of Gpx4 expression in each group. Overexpression of Nrf2 significantly reduced GPX4 depletion (×200). (d). Western blot assays for expression of related factors among the different groups. Transfection with Nrf2 viral vectors increased the expression of Nrf2 in the nucleus ().

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