Oridonin Relieves Angiotensin II-Induced Cardiac Remodeling via Inhibiting GSDMD-Mediated Inflammation

Figure 1

Oridonin can alleviate Ang II-induced myocardial hypertrophy. (a) Structural formula of oridonin. (b, c) The effect on cell viability after incubation of different concentrations of oridonin in H9c2 cells and neonatal rat cardiomyocytes. (d, f) Pretreated H9c2 cells and neonatal rat cardiomyocytes with Ori (1 and 2.5 μM) for 1 h before daylong incubation with 1 μM Ang II. Lysates were analyzed by western blotting for the expression of proteins associated with hypertrophy, and the corresponding semiquantitative statistical graph is shown. (e) The mRNA level of MyHC standardized by β-actin in H9c2 cells. (g) The mRNA level of MyHC in neonatal rat cardiomyocytes. (h) Analysis of the levels of MyHC in H9c2 cells when treated with Ori only. (i, j) Rhodamine-phalloidin staining to determine changes in cell size. 200x magnification; . compared with control; ^# compared with Ang II.

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