MicroRNA-325-3p Targets Human Epididymis Protein 4 to Relieve Right Ventricular Fibrosis in Rats with Pulmonary Arterial Hypertension

Figure 3

miR-325-3p-targeted regulation of HE4 to inhibit myocardial fibroblast fibrosis. (a) Starbase was applied to predict miR-325-3p and HE4 binding sites. (b) Dual-luciferase reporter assay experiments proved that miR-325-3p targeted HE4. (c) miR-325-3p and HE4 expressions were assessed by qRT-PCR. (d) Expression of Acta2 was measured by qRT-PCR. (e) α-SMA expression was detected by IF (×400, 25 μm). (f) qRT-PCR and Western blot were utilized to detect collagen I, collagen III, MMP2, and MMP9 levels. vs. control; ^# vs. NC mimics.

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