CircTRRAP Knockdown Has Cardioprotective Function in Cardiomyocytes via the Signal Regulation of miR-370-3p/PAWR Axis

Figure 4

CircTRRAP/miR-370-3p signal axis regulated the hypoxia-stimulated cell injury. AC16 cells with hypoxic treatment (24 h) were transfected with si-NC, si-circTRRAP, si-circTRRAP+in-miR-NC, or si-circTRRAP+in-miR-370-3p. (a, b) Cell viability (a) and proliferation (b) were assessed via CCK-8 assay and EdU assay. (c–e) Cell apoptosis was measured via flow cytometry (c), western blot (d), and caspase-3 activity assay (e). (f–h) Inflammatory response was analyzed via ELISA. (i–k) Oxidative stress was assessed via the commercial kits. The experiments were repeated for three times with three paralleled samples. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)