CircTRRAP Knockdown Has Cardioprotective Function in Cardiomyocytes via the Signal Regulation of miR-370-3p/PAWR Axis

Figure 6

Overexpression of miR-370-3p protected AC16 cells against the hypoxia-induced injury by inhibiting PAWR level. AC16 cells under the hypoxic condition (24 h) were transfected with miR-NC, miR-370-3p, miR-370-3p + pcDNA, or miR-370-3p + PAWR. (a, b) CCK-8 (a) and EdU assay (b) were used to determine cell viability (a) and proliferation (b). (c–e) Flow cytometry (c), western blot (d), and caspase-3 activity assay (e) were applied for apoptosis analysis. (f–h) ELISA was applied for the measurement of inflammatory cytokines. (i–k) The detection kits were used for the evaluation of oxidative stress. The experiments were repeated for three times with three paralleled samples. .

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