Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b

Figure 2

APD and Kv channel expression in response to P4 treatment. Changes in the HR (a) and QTc interval (b) caused by P4 treatment during pregnancy (pregnant and pregnant subjects who received P4 ). The pregnant mice were injected daily with P4 (50 mg/kg/d) or sesame oil during gestation for 18 days. Age-matched nonpregnant mice injected with sesame oil served as controls (c). HR (d) and QTc interval (e) of pregnant mice with or without P4 injections. (f) Representative recordings of the APs in cardiomyocytes stimulated by the rectangular pulse from pregnant mice and pregnant mice that received P4 injections. (g–i) Comparisons of APD₃₀, APD₅₀, and APD₉₀ between pregnant mice with and without P4 injections. (j) qRT–PCR analysis of Kv channels in ventricles from pregnant mice and pregnant mice treated with P4. , , and ( from 4-6 mice per group). Comparisons of data were conducted by Student’s test or one-way ANOVA followed by the Bonferroni post hoc test. P: pregnant group; P4: progesterone; HR: heart rate; QTc: corrected QT; APD: action potential duration.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)