Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b

Figure 3

Whole-cell Kv currents and Kv channel expression in response to P4 treatment in the H9C2 myocyte line. The H9C2 cells were exposed to 1 μM or 5 μM P4 and cultured for 48 h. The H9C2 cells without treatment and cultured under normal conditions were used as controls (Ctl). (a) Representative recordings of whole-cell voltage clamp experiments in control, 1 μM P4-, and 5 μM P4-treated H9C2 cells for 48 h. (b) The current–voltage (IV) curve from each group was analyzed at a steady state of the voltage-dependent currents (150 to 180 ms). (c) The current density in each group was compared at +60 mV. and vs. Ctl ( cells per group). Comparisons of data were conducted by a one-way ANOVA followed by the Bonferroni post hoc test. (d) qRT–PCR analysis of Kv channels in the H9C2 cells cultured under 1 μM and 5 μM P4 conditions ( individual experiments per group). , , and vs. Ctl. Comparisons of data were conducted by a one-way ANOVA followed by the Bonferroni post hoc test. P4: progesterone.

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