Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b

Figure 4

Effects of RU486 on the P4-regulated expression of Kv2.1. The H9C2 cells were exposed to 1 μM P4 and 5 μM P4 and cultured with/without 5 μM RU486 for 48 h. The H9C2 cells without any treatment and cultured under standard conditions were used as controls (Ctl). (a and c) qRT–PCR analysis of Kv2.1 in the H9C2 cells cultured under 1 μM and 5 μM P4 conditions with/without 5 μM RU486 application. (b and d) WB analysis of Kv2.1 in the H9C2 cells cultured under 1 μM and 5 μM P4 conditions with/without 5 μM RU486 application. and vs. Ctl ( individual experiments). Comparisons of data were conducted by a one-way ANOVA followed by the Bonferroni post hoc. P4: progesterone; RU486: an antagonist of P4; qRT–PCR: quantitative real-time polymerase chain reaction; WB: western blot.

(a)

(b)

(c)

(d)