KCa3.1 Promotes Proinflammatory Exosome Secretion by Activating AKT/Rab27a in Atrial Myocytes during Rapid Pacing

Figure 6

Rapid pacing activates the KCa3.1/AKT/Rab27a signaling pathway. (a) Representative gel bands showing the effects of BAPTA, GSK690693, and si-Rab27a on rapid-pacing HL-1 cells. (b) Representative gel bands showing the effects of different concentrations of SC79. (c–g) The levels of KCa3.1, p-AKT/AKT, Rab27a, TSG101, and CD81 after treatment with BAPTA, GSK690693, and si-Rab27a. (h–j, l) The levels of p-AKT/AKT, Rab27a, TSG101, and CD81 after treatment with different concentrations of SC79. (k, m) Representative images of Rab27a after treatment with BAPTA, GSK690693, and si-Rab27a, as reflected by immunofluorescence analysis. The data are presented as the of three biological replicates. , , and vs. control group; ^# and ^## vs. pacing group.

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