KCa3.1 Promotes Proinflammatory Exosome Secretion by Activating AKT/Rab27a in Atrial Myocytes during Rapid Pacing

Figure 7

KCNN4 overexpression promotes exosome secretion. (a) Representative gel bands showing the effects of TRAM-34 administration on KCNN4-overexpressing HL-1 cell. (b) Representative gel bands showing the effects of BAPTA, GSK690693, and si-Rab27a on KCNN4-overexpressing HL-1 cell. (c–g). The levels of KCa3.1, p-AKT/AKT, Rab27a, TSG101, and CD81 in KCNN4-overexpressing HL-1 cell after TRAM-34 administration. (h, i) Mean exosome diameter and concentration, as shown by the Zeta View System. The exosomes were extracted from the same volume of supernatant from cells of equal density. (j–n) The levels of KCa3.1, p-AKT/AKT, Rab27a, TSG101, and CD81 in KCNN4-overexpressing HL-1 cell after treatment with BAPTA, GSK690693, and si-Rab27a. The data are presented as the mean ± SD of three biological replicates. , , and indicate , 0.01, and 0.001, respectively.

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