Cardioprotection of mAb2G4/ODN/lip on Myocardial Ischemia-Reperfusion Injury via Inhibiting the NF-κB Signaling Pathway

Figure 7

Analysis of the expression of inflammatory proteins by western blot in vitro. Results of western blot in the expression of caspase-1 P20 protein, procaspase-1 protein, p-NF-κB, and NLRP3 protein in vitro (a). Effects of ODN/lip and mAb2G4/ODN/lip on the expression of caspase-1 P20 protein (b), procaspase-1 protein (c), p-NF-κB (d), and NLRP3 protein (e) in H/R ventricular myocyte model. Control group (cells were cultured in the normal incubating condition), H/R group (cells were subjected to hypoxia for 2 hours and reoxygenation 1-hour treatment), LO group (treated with 2 μg ODN/lip), HO group (treated with 4 μg ODN/lip), LM group (treated with 2 μg mAb2G4/ODN/lip), and HM group (treated with 4 μg mAb2G4/ODN/lip). Data were expressed as . ^ns, , , , and . H/R group vs. control group; LO group, HO group, LM group, and HM group vs. H/R group; LO group vs. HO group; LM group vs. HM group; LO group vs. LM group.

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