E-Selectin/AAV Gene Therapy Promotes Myogenesis and Skeletal Muscle Recovery in a Mouse Hindlimb Ischemia Model

<div>E-sel/AAV enhances proliferation of myogenic precursors in ischemic muscle. (a) Representative immunofluorescence images and (b) quantification of MyoD and Ki-67 expression demonstrating increased number of MyoD<sup>+</sup>/Ki-67+ myogenic precursors (white arrow) in ischemic gastrocnemius muscle treated with E-sel/AAV compared to LacZ/AAV (<svg height="8.8423pt" id="M82" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -8.6359 32.2343 8.8423" width="32.2343pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M822 650H589L583 622C660 617 677 607 674 561C672 534 664 481 647 390L600 137H596L273 650H126L120 622C176 620 194 615 207 594C221 571 225 557 214 504L161 257C141 166 129 112 121 85C108 42 83 30 29 28L23 0H260L266 28C193 33 173 42 176 89C178 122 186 172 202 255L256 527H259L583 -8H612L690 390C708 481 720 535 728 558C744 603 756 619 816 622L822 650Z"></path></g><g transform="matrix(.013,0,0,-0.013,14.542,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,25.805,0)"><path d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z"></path></g></svg> per group). In comparison, few MyoD<sup>+</sup> or Ki-67<sup>+</sup> cells are identified in non-treated, non-ischemic muscle from either group. Scale bars represent 50 <i>μ</i>m. 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Cardiovascular Therapeutics

fig6

Figure 6

Figure 6: E-Selectin/AAV Gene Therapy Promotes Myogenesis and Skeletal Muscle Recovery in a Mouse Hindlimb Ischemia Model 