miR-135a Regulates Atrial Fibrillation by Targeting Smad3

Figure 3

miR-135a directly regulated Smad3 expression. (a) TargetScan database predicted interactions between miR-135a and their binding site in the Smad3 3-UTR; (b) fragments of the Smad3 3-UTR were cloned into pmirGLO reporter plasmids. The mutated nucleotides are marked in red. HEK293 cells were transfected with miR-135a mimics (c) or inhibitors (d) along with the indicated reporter plasmids for 24 h. The relative luciferase activities were analyzed. ().

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