Valsartan Attenuated Homocysteine-Induced Impaired Autophagy and ER Stress in Human Umbilical Vein Endothelial Cells

<div>GSK-3<i>β</i> inhibitor TDZD-8 treatment reversed Hcy-induced impaired autophagy, ER stress, and apoptosis in HUVECs. (a, b) Representative immunoblots and corresponding densitometry analysis of LC3B, Beclin1, and p62 protein in Hcy-treated HUVECs with or without TDZD-8. (c, d) Representative immunoblots and corresponding densitometry analysis of BiP, IRE1<i>α</i>, XBP1, ATF4, and CHOP protein in Hcy-treated HUVECs with or without TDZD-8. (e, f) Representative immunoblots and corresponding densitometry analysis of Bax, Bcl2, and cleaved-caspase 3 protein in Hcy-treated HUVECs with or without TDZD-8. 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238 395C212 395 165 379 127 364L153 550Z"></path></g></svg> compared with the CTL group; <sup>#</sup><svg height="9.2729pt" id="M24" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 44.8393 9.2729" width="44.8393pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,22.921,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 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Cardiovascular Therapeutics

fig6

Figure 6

Figure 6: Valsartan Attenuated Homocysteine-Induced Impaired Autophagy and ER Stress in Human Umbilical Vein Endothelial Cells 