Attenuating Atherosclerosis through Inhibition of the NF-κB/NLRP3/IL-1β Pathway-Mediated Pyroptosis in Vascular Smooth Muscle Cells (VSMCs)

Figure 1

VSMCs (CRL-1999) were treated with Res (10 μM) for the indicated time. (a) Effects of different concentrations of Res on proliferation activity of VSMCs. Data are expressed as mean ± SEM. , vs. 0 μmol/L. (b) Calcium deposition in VSMCs was assessed by alizarin red S staining (positive staining: red; scale bar = 100 μm). (c) The mRNA level of Runx2 was determined by qPCR. Data are expressed as . vs. control group; vs. AS group. (d) The protein levels of BMP2 and Runx2 were determined by western blotting. (e, f) Quantification of the results shown in (c). Data are expressed as . vs. control group; , vs. AS group. (g) Representative immunofluorescent staining images for Runx2 (green) (magnification: ×400, scale bar = 20 μm).

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