DUSP22 Ameliorates Endothelial-to-Mesenchymal Transition in HUVECs through Smad2/3 and MAPK Signaling Pathways

Figure 5

DUSP22 impacts the MAPK signaling pathway. sh-DUSP22 activates the MAPK signaling pathway. The (a) protein representative blots and (b) quantitative results of the p-JNK/JNK and p-ERK/ERK. vs. sh-NC. ^# vs. sh-NC+TGF-β. OE^DUSP22 inactivates the MAPK signaling pathway. The (c) protein representative blots and (d) quantitative results of the p-JNK/JNK and p-ERK/ERK. vs. OE^NC. ^## vs. OE^NC+TGF-β. After adding with JNK inhibitor SP600125, the (e) mRNA expression changes and the (f) protein representative blots and (g) quantitative results of the phenotypic molecule of EndMT. After adding with ERK inhibitor ravoxertinib, the (h) mRNA expression changes and the (i) protein representative blots and (j) quantitative results of the phenotypic molecule of EndMT. ¹ vs. sh-NC. ² vs. sh-NC+TGF-β. ³ vs. sh-DUSP22. ⁴ vs. sh-DUSP22+TGF-β.

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