Changes in Activity and Kinetic Properties of the Proteasome in Different Rat Organs during Development and Maturation

Figure 2

Michaelis-Menten constants (𝐾_𝑚) of the proteasome in rat brain with age. Michaelis-Menten constants of the proteasome in rat brain plotted against age: 𝐾_𝑚-values were obtained as described in Figure 1. (a) Chymotrypsin-like activity: 𝑅²=0.024, 𝑃=.291. (b) Trypsin-like activity: 𝑅²=0.189, 𝑃=.002. (c) Peptidylglutamyl peptidase activity: 𝑅²=0.060, 𝑃=.094. A 𝑃-value of less than  .05 was considered statistically significant.

(a) Chymotrypsin-like activity (LLVY)

(b) Trypsin-like activity (VGR)

(c) Peptidylglutamyl-peptidase activity (LLE)