RGD-Hydrogel Improves the Therapeutic Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Phosgene-Induced Acute Lung Injury in Rats

Figure 1

The cytotoxicity of RGD-hydrogel in bone marrow-derived MSCs. (a) SEM image of RGD-hydrogel surface (Bar = 50 μm). (b) The morphology of MSCs cultured in conventional medium and RGD-hydrogel medium under a light microscope (Bar = 200 μm). (c) The viability of MSCs after culturing with different concentrations of RGD-hydrogel (1 : 0, 1 : 1, 1 : 2, 1 : 3, and 1 : 4 v/v) for 1 day, 3 days, and 7 days using CCK-8 assay kit. (d) The LDH release of MSCs after culturing with different concentrations of RGD-hydrogel (1 : 0, 1 : 1, 1 : 2, 1 : 3, and 1 : 4 v/v) for 1 day, 3 days, and 7 days using LDH assay kit. (e) The biocompatibility and degradability of RGD-hydrogel in vivo after injection with 50 μL RGD-hydrogel for 1 day, 3 days, and 7 days (Bar = 200 μm). :  < 0.05, :  < 0.01, compared with the control group. #:  < 0.05, ##:  < 0.01, compared with the 1 : 3 v/v group.

(a)

(b)

(c)

(d)

(e)