The Protective Effect of Ursolic Acid on Unilateral Ureteral Obstruction in Rats by Activating the Nrf2/HO-1 Antioxidant Signaling Pathway

Figure 4

Ursolic acid reduces the apoptosis of renal tubular epithelial cells after unilateral ureteral obstruction in rats TUNEL staining is used to mark apoptotic cells; DAPI staining is used to mark normal cell nuclei; MERGE represents the combination of the two; observed under a fluorescence microscope, normal cell nuclei are blue, and apoptotic cell nuclei are green; (a) the apoptosis of renal tubular epithelial cells on the 8th day after operation in each group of rats; (b) the Western blot of caspase-3 and caspase-8 apoptotic proteins in each group of rats; (c) the statistical graph of the apoptosis index of renal tubular epithelial cells in each group (the percentage of the number of apoptotic cells in the total number of tubular epithelial cells is used as the apoptotic index (AI)) (mean ± standard deviation), n = 6); (d) the statistical graph of the apoptotic protein caspase-3 mRNA in each group; (e) the statistical graph of the apoptotic protein caspase-8 mRNA in each group; (f) the statistical graph of the expression level of the apoptotic protein Caspase-3; (g) the statistical graph of the expression level of the apoptotic protein caspase-8; means ; means ; means ; means .

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