[Retracted] LncRNA BC083743 Silencing Exacerbated Osteoporosis by Regulating the miR-103-3p/SATB2 Axis to Inhibit Osteogenic Differentiation

Figure 3

miR-103-3p was a downstream effector of BC083743. (a) Starbase online database predicts miR-103-3p as a target miRNA for BC083743. (b) Transfection of miR-103-3p mimics or NC mimics into hBMSCs cells. The levels of miR-103-3p were detected by QRT-PCR. (c) The relationship between BC083743 and miR-103-3p was verified using a dual-luciferase assay. (d) The RNA pull-down assay verified the binding between BC083743 and miR-103-3p. (e) QRT-PCR detection of miR-103-3p expression after BC083743 overexpression knockdown. (f) QRT-PCR was used to detect serum miR-103-3p levels in OP patients and normal controls. (g) QRT-PCR detection of miR-103-3p levels in hBMSCs as osteogenic differentiation time was prolonged. , , compared with miR-NC/si-NC/pcDNA3.1-NC.

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