Olfm4 Is Highly Expressed in HCC Patients and as a Biomarker and Therapeutic Target for HCC

Figure 5

OLFM4 regulated liver cancer cell proliferation in HepG2 cells. (a) siRNAs against OLFM4 successfully inhibited the mRNA expression of OLFM4 in HepG2 cells measured by qRT-PCR assay (); (b) siRNAs against OLFM4 successfully inhibited the protein level of OLFM4 in HepG2 cells measured by western blot assay; (c) siRNAs against OLFM4 could inhibit the proliferation of HepG2 cells (, ); (d) OLFM4 plasmid transfection could overexpress the gene in HepG2 cells detected by qRT-PCR assay (); (e) OLFM4 plasmid transfection could upregulate the protein level of OLFM4 in HepG2 cells detected by western blot assay; and (f) OLFM4 plasmid could affiliate the proliferation of HepG2 cells ().

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