A New CYP2E1 Inhibitor, 12-Imidazolyl-1-dodecanol, Represents a Potential Treatment for Hepatocellular Carcinoma

Figure 3

Regulation of cell-cycle progression. (a) Representation of each cell-cycle phase with increasing I-ol concentrations. A phase shift was observed at an I-ol concentration of 30 μM. Specifically, we observed disappearance of cells in the S phase, a significant increase of S-phase arrest, and a large increase in the percentage of cells in G2/M phase and sub-G1 phase at the highest concentration. (b) Summary of the percentages of individual cells in each phase. (c) Representative scatter chart, where the y-axis represents the EdU-specific fluorescence on a logarithmic scale and the x-axis represents the cell cycle-specific fluorescence on a linear scale. The results after 48-hour incubation with I-ol are shown. After 72 or 96 h comparable results were obtained, those data are not shown to avoid redundancy. The results are shown as the mean ± SD. Statistical calculations were performed based on two-way mixed ANOVA, followed by Bonferroni’s post hoc test. In all cases, the significance levels were (significant), (highly significant), or (very highly significant).

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