Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples

Table 1

Monoclonal antibodies and antigen retrieval protocol used for the Leica IHC detection system.

Antibodies	Company and catalog number	Specific to	Antigen retrieval (buffer, temperature, Duration)
H. pylori mAb	MBL, C369-3	H. pylori LPS	ER2, 100°C, 20 min
CagA mAb	SCB, sc-28368	H. pylori CagA	ER2, 100°C, 20 min
EPIYA-C mAb	–	EPIYA-C motif	ER1, 100°C, 20 min
EPIYA-D mAb	–	EPIYA-D motif	ER2, 100°C, 20 min

IHC: immunohistochemistry, mAb: monoclonal antibody, LPS: lipopolysaccharide. MBL : Medical and Biological Laboratories, Tokyo, Japan, SCB : Santa Cruz Biotechnology, CA, USA, ER2, ER1: antigen retrieval with BOND Epitope Retrieval Solution 2 or Solution 1 (#AR9960 or #AR9961, Leica Microsystems Inc., Tokyo, Japan).