Pumilio RNA-Binding Family Member 1 Plays a Promoting Role on Pancreatic Cancer Angiogenesis

Figure 5

Effect of pancreatic cancer cells overexpressing or silencing PUM1 on tube formation ability of HUVECs. To construct a transwell co-culture system, MIA PaCa-2 and PANC-1 cells stably overexpressing PUM1 (ov-PUM1), silencing PUM1 (sh-PUM1), and their negative control cells (ov-NC and sh-NC) were cultured in the upper chamber of a transwell insert, and HUVECs were cultured in the lower chamber. Matrigel-based tube formation assay was performed to assess tube formation ability of HUVECs. Left panel is representative images, and right panel is the statistical result of three independent experiments. ^∗ < 0.05, ov-PUM1 vs. ov-NC; ^  < 0.05, sh-PUM1 vs. sh-NC.