SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway

Figure 3

NMT1 modulated GC cell migration and invasion. (a) Migration rate of AGS cells at 48 h was evaluated through wound healing assay after transfection of NMT1 overexpression plasmid. (b) Migration rate of SNU-5 cells at 48 h was evaluated through wound healing assay after transfection of shNMT1. (c) Invasion rate of AGS cells at 48 h was determined by transwell assay after transfection of NMT1 overexpression plasmid. (d) Invasion rate of SNU-5 cells at 48 h was determined by Transwell assay after transfection of shNMT1. ^∗∗ < 0.01, ^∗∗∗ < 0.001 vs. NC group; ^∧∧ < 0.01, ^∧∧∧ < 0.001 vs. shNC group. All experiments were repeated independently at least three times. Data were performed as the means ± standard deviation. NMT1, N-myristoyltransferase 1; GC, gastric cancer; shNMT1, short hairpin RNA against NMT1; NC, negative control.

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