SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway

Figure 6

SPI1 regulated NMT1 to mediate GC cell migration and invasion. (a) Migration rate of AGS cells at 48 h was evaluated through wound healing assay after transfection of NMT1 overexpression plasmid and shSPI1. (b) Migration rate of SNU-5 cells at 48 h was evaluated through wound healing assay after transfection of shNMT1 and SPI1 overexpression plasmid. (c) Invasion rate of AGS cells at 48 h was determined by Transwell assay after transfection of NMT1 overexpression plasmid and shSPI1. (d) Invasion rate of SNU-5 cells at 48 h was determined by Transwell assay after transfection of shNMT1 and SPI1 overexpression plasmid. ^∗ < 0.05, ^∗∗∗ < 0.001 vs. NC + shNC group; ^∧∧∧ < 0.001 vs. NMT1+shNC group; ^## < 0.01, ^### < 0.001 vs. NC + shSPI1 group; ⁺⁺⁺ < 0.001 vs. shNMT1+NC group; ^△△△ < 0.001 vs. shNC + SPI1 group. All experiments were repeated independently at least three times. Data were performed as the means ± standard deviation. NMT1, N-myristoyltransferase 1; GC, gastric cancer; shNMT1, short hairpin RNA against NMT1; NC, negative control; shNC, shRNA negative control.

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