Review of 16S and ITS Direct Sequencing Results for Clinical Specimens Submitted to a Reference Laboratory

Table 2

Amplification and identification rates for direct ITS testing.

Specimen type	Number of positive specimens	Number of negative specimens	Percent positive	Total number of specimens
Fresh tissue
Microscopy positive	6	13	31.6%	19
Microscopy negative	0	20	0.0%	20
No microscopy result	0	4	0.0%	4
Total	6	37	14.0%	43
FFPE tissue
Microscopy positive	2	18	10.0%	20
Microscopy negative	2	8	20.0%	10
No microscopy result	1	2	33.3%	3
Total	5	28	15.2%	33
Sterile fluids
Microscopy positive	1	4	20.0%	5
Microscopy negative	1	19	5.0%	20
No microscopy result	1	8	11.1%	9
Total	3	31	8.8%	34
Total for all specimens
Microscopy positive	9	35	20.5%	44
Microscopy negative	3	47	6.0%	50
No microscopy result	2	14	12.5%	16
Total	14	96	12.7%	110

Formalin fixed paraffin embedded.