A New Functional Model for Prediction of Chaperone Activity of the Recombinant M. tb Acr (α-Crystallin) Using Insulin as Substrate

Table 2

Chaperone activity of (H) and (G) versus mole ratio at 60°C and 37°C. Polynomial best fit has been plotted in MS excel with XY scatter function in charts along with trendline with equation and R² value.

Mole ratio of Acr (H) sample to insulin at 60°C	% inhibition
0	0
0.033	25
0.066	30
0.113	60
0.226	95
Mole ratio of Acr (H) sample to insulin at 37°C	% inhibition
0	0
0.05	50
0.09	60
0.15	67
0.25	98
Mole ratio of Acr (G) sample, run 1, pool 1 to insulin at 37°C	% inhibition
0	0
0.051	26
0.059	30
0.076	33
0.102	91
Mole ratio of Acr (G) sample, run 1, pool 2 to insulin at 37°C	% inhibition
0	0
0.017	67
0.034	84
0.051	99
Mole ratio of Acr (G) sample, run 2 to insulin at 37°C	% inhibition
0	0
0.017	12
0.025	47
0.034	79
0.068	87