Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis

Figure 1

The specificity of EvaGreen multiplex real-time PCR protocol for the eight bacteria. (a) Results obtained for each bacterium when tested by multiplex real-time PCR protocol. (b) Each distinct peak corresponded to a specific bacterial standard strain DNA amplified by multiplex real-time PCR protocol, where nontemplate negative controls (NTC) did not show any peaks.

(a)

(b)