Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis

Figure 4

(a)–(h) Standard curves were obtained by plotting the fluorescence intensity (y-axis) values against the log₁₀ of the bacterial concentration (x-axis) of the eight respective standard bacterial strains. The curve equations and R² are as follows: y = 6.53x − 5.52, R² = 0.988 for A. baumannii (a); y = 7.15x − 22.87, R² = 0.987 for E. coli (b); y = 5.92x − 14.08, R² = 0.980 for K. pneumoniae (c); y = 3.66x + 1.02, R² = 0.965 for H. influenzae (d); y = 1.42x + 4.19, R² = 0.947 for S. pneumoniae (e); y = 5.81x − 6.37, R² = 0.998 for S. aureus (f); y = 4.15x − 13.39, R² = 0.995 for P. aeruginosa (g); y = 1.08x + 1.34, R² = 0.943 for M. tuberculosis (h).

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