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| Virulence factors | Genes | Primer name | Sequence (5′–3′) | Product length (bp) |
|
| Hemolysin | hla | hla-F | ATGAAAACACGTATAGTCAGCTCAGTAACAAC | 960 |
| hla-R | TTAATTTGTCATTTCTTCTTTTTCCCAATCGA |
| hlb | hlb-F | AAATTAGAGCTGAACAAATGAAAGAAATCAG | 825 |
| hlb-R | TTTCGCAATTGAATTTGATTGAGGGTCCC |
| hld | hld-F | ATGGCACAAGATATCATTTCAACAATCAGTG | 81 |
| hld-R | TTATTTTTTAGTGAATTTGTTCACTGTGTCG |
| hlgA | hlgA-F | ATGATTAAAAATAAAATATTAACAGCAACTTTAGCAGTT | 860 |
| hlgA-R | TTACTTAGGTGTGATGCTTTTAATTTTTACTTCATGTG |
| hlgB | hlgB-F | ATGAAAATGAATAAATTAGTCAAATCATCCGTTGC | 792 |
| hlgB-R | TTACTGTAATTTTAGATTTTTTAGCGCCATCTTG |
| hlgC | hlgC-F | GCTTAAAAATAAAATATTAACTACAACTTTATCTGTGAGC | 720 |
| hlgC-R | TCAATTCTGTCCTTTCACCTTGATTTCATGAG |
| Leukocidin | lukE | lukE-F | TTGTCAGTAGGACTGATTGCACCTTTAGC | 906 |
| lukE-R | TTAATTATGTCCTTTCACTTTAATTTCGTGTGTT |
| lukM | lukM-F | GTTTAAGAGAAAATTATTAGTTACAACTTTGTCGC | 864 |
| lukM-R | GTTGTGCCCCTTTACTTTAATTTCGTG |
| lukF-PV | lukF-PV-F | ATGAAAAAAATAGTCAAATCATCAGTTGTTACATCA | 978 |
| lukF-PV-R | AGCTCATAGGATTTTTTTCCTTAGATTGAG |
| lukS-PV | lukS-PV-F | GGTCAAAAAAAGACTATTAGCTGCAACATTG | 939 |
| lukS-PV-R | TCAATTATGTCCTTTCACTTTAATTTCATGAGTTTTCC |
| Invasive proteases | splB | splB-F | GAACAAAAACGTAGTCATCAAGAGTTTAGCAGC | 723 |
| splB-R | CTATGTTTTCTGCAATGAATTTTTTAATTTCTGGTGT |
| sspA | sspA-F | ATGAAAGGTAAATTTTTAAAAGTTAGTTCTTTATTCGT | 783 |
| sspA-R | ATCTTCAATATTTTGTTTTAAGAAGTTGCGTACA |
| sspB | sspB-F | GTAAATCTAGAGTATTCAATATTATCAGCATCATAATGG | 792 |
| sspB-R | AACCTATCATTGAACCATACCAGTTATAATCA |
| sspC | sspC-F | GTATCAACTACAATTTATAAATTTAGTTTACGACACAACC | 330 |
| sspC-R | CTAAGCGCTCATAAACGATTGGGCGC |
| Hyaluronidase | hysA | hysA-F | ATGACATATAGAATGAAGAAATGGCAAAAATTATCCACC | 936 |
| hysA-R | TAATTCAAAGCGCACGCCGGATTCATTAGA |
| Staphylokinase | sak | sak-F | ATGCTCAAAAGAGGTTTATTATTTTTAACTGTTTT | 492 |
| sak-R | ATTTCTTTTCTATAACAACCTTTGTAATTAAGTTG |
| Lipase | lip | lip-F | GTAGATTATGGTGCAGCACATGCAGCAAAATATGG | 921 |
| lip-R | AGCTTTTCAGTTTTCACTAAATCGTCTGCT |
| Nuclease | nuc | nuc-F | AAGAGGTTTTTCTTTTTCGCTACTAGTTGC | 537 |
| nuc-R | CTCCAAATATTTAATTTCTGTTGTTTAGCTTT |
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