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Canadian Journal of Infectious Diseases and Medical Microbiology
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Canadian Journal of Infectious Diseases and Medical Microbiology
/
2022
/
Article
/
Tab 1
/
Research Article
Molecular Assessment of Domain I of Apical Membrane Antigen I Gene in
Plasmodium falciparum
: Implications in
Plasmodium
Invasion, Taxonomy, Vaccine Development, and Drug Discovery
Table 1
Primers used to genotype the
Plasmodium falciparum
AMA1 gene.
Target
Primers
Sequence 5′-3′
Whole PfAMA1 gene
First round of PCR
Forward
CCGGATCCCCTTTGAGTTTACATATATG
Reverse
AAATTCTTTCTAGGGCAAAC
Domain I of PfAMA1
Second round of PCR
Forward
GGAACTCAATATAGACTTCC
Reverse
AAATTCTTTCTAGGGCAAAC