Research Article

Molecular Assessment of Domain I of Apical Membrane Antigen I Gene in Plasmodium falciparum: Implications in Plasmodium Invasion, Taxonomy, Vaccine Development, and Drug Discovery

Table 1

Primers used to genotype the Plasmodium falciparum AMA1 gene.

TargetPrimersSequence 5′-3′

Whole PfAMA1 gene
First round of PCR
ForwardCCGGATCCCCTTTGAGTTTACATATATG
ReverseAAATTCTTTCTAGGGCAAAC
Domain I of PfAMA1
Second round of PCR
ForwardGGAACTCAATATAGACTTCC
ReverseAAATTCTTTCTAGGGCAAAC