Research Article
Decreased Susceptibility of Shigella Isolates to Azithromycin in Children in Tehran, Iran
| | Target gene | Primer sequence (5′ ⟶ 3′) | Product size (bp) | Annealing temperature (°C) | Reference |
| | mph(A) | F: GTGAGGAGGAGCTTCGCGAG | 403 | 59 | Rahman et al. [28] | | R: TGCCGCAGGACTCGGAGGTC | | mph(B) | F: GATATTAAACAAGTAATCAGAATAG | 494 | 49 | Rahman et al. [28] | | R: GCTCTTACTGCATCCATACG | | erm(A) | F: TCTAAAAAGCATGTAAAAGAAA | 533 | 47 | Rahman et al. [28] | | R: CGATACTTTTTGTAGTCCTTC | | erm(B) | F: GAAAAAGTACTCAACCAAATA | 639 | 43 | Rahman et al. [28] | | R: AATTTAAGTACCGTTACT | | erm(C) | F: TCAAAACATAATATAGATAAA | 642 | 43 | Rahman et al. [28] | | R: GCTAATATTGTTTAAATCGTCAAT | | erm(F) | F: CGACACAGCTTTGGTTGAAC | 302 | 52 | Liu et al. [29] | | R: GGACCTACCTCATAGACAAG | | erm(T) | F: CATATAAATGAAATTTTGAG | 369 | 42 | Liu et al. [29] | | R: ACGATTTGTATTTAGCAACC | | erm(X) | F: GAGATCGGRCCAGGAAGC | 488 | 54 | Liu et al. [29] | | R: GTGTGCACCATCGCCTGA | | ere(A) | F: GCCGGTGCTCATGAACTTGAG | 420 | 55 | Rahman et al. [28] | | R: CGACTCTATTCGATCAGAGGC | | ere(B) | F: TTGGAGATACCCAGATTGTAG | 537 | 50 | Rahman et al. [28] | | R: GAGCCATAGCTTCAACGC | | mef(A) | F: AGTATCATTAATCACTAGTGC | 345 | 48 | Rahman et al. [28] | | R: TTCTTCTGGTACTAAAAGTGG | | msr(A) | F: GCACTTATTGGGGGTAATGG | 384 | 51 | Rahman et al. [28] | | R: GTCTATAAGTGCTCTATCGTG | | omp(A) | F: ATGAAAAAGACAGCTATCGCG | 187 | 54 | Gomes et al. [30] | | R: CACCAAAAGCACCAGCGCCCA | | omp(W) | F: GTTAACAGTGGCGGGTTTGGC | 154 | 56 | Gomes et al. [30] | | R: CACGCTGAATCCACCCAGACT |
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bp, base pair; F, forward primer; R, reverse primer.
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