Rapid Identification of Drug-Resistant Tuberculosis Genes Using Direct PCR Amplification and Oxford Nanopore Technology Sequencing

Table 1

Multiplex polymerase chain reaction primer sequences.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Amplicon size (bp)
rpoB	CTTGCACGAGGGTCAGACCA	ATCTCGTCGCTAACCACGCC	543
katG	AACGACGTCGAAACAGCGGC	GCGAACTCGTCGGCCAATTC	455
inhA	TGCCCAGAAAGGGATCCGTCATG	ATGAGGAATGCGTCCGCGGA	455
eis	GCGTAACGTCACGGCGAAATTC	GTCAGCTCATGCAAGGTG	567
rrs	GTCAACTCGGAGGAAGGTGG	GTCCGAGTGTTGCCTCAGG	516
gyrB	AAGACCAAGTTGGGCAACAC	CTGCCACTTGAGTTTGTACA	609
gyrA	AGACACGACGTTGCCGCCTG	CTGACCCGTTGGCCAGCAGG	530