Research Article

Rapid Identification of Drug-Resistant Tuberculosis Genes Using Direct PCR Amplification and Oxford Nanopore Technology Sequencing

Table 2

Percentage (%) identify of consensus sequences to Sanger sequencing results.

SampleropBkatGgyrAgyrBrrseis
NL/SLa (bp/bp)Identify (%)NL/SL (bp/bp)Identify (%)NL/SL (bp/bp)Identify (%)NL/SL (bp/bp)Identify (%)NL/SL (bp/bp)Identify (%)NL/SL (bp/bp)Identify (%)

Y12543/454100.00455/363100.00530/441100.00b567/477100.00
Y50543/456100.00530/443100.00
Y76543/459100.00530/438100.00609/521100.00
Y80543/453100.00455/355100.00530/442100.00516/428100.00
Y83543/461100.00530/438100.00
Y88543/454100.00530/444100.00
Y105543/462100.00455/367100.00530/444100.00609/524100.00
Y143530/430100.00
Y145530/439100.00
Y159530/440100.00
Y183530/437100.00
Y189530/436100.00
Y170543/456100.00530/439100.00
Y191530/437100.00
Y208530/437100.00
Y221530/438100.00
Y252543/453100.00530/439100.00609/512100.00
Y254530/445100.00
Y256543/455100.00455/366100.00530/427100.00
Y281530/440100.00

aNL/SL, Nanopore assembly length/Sanger sequencing length; b, null.