Differential Infectivity of Human Neural Cell Lines by a Dengue Virus Serotype-3 Genotype-III with a Distinct Nonstructural Protein 2A (NS2A) Amino Acid Substitution Isolated from the Cerebrospinal Fluid of a Dengue Encephalitis Patient

Figure 4

Infectivity of DENV-3 was measured by the expression of viral antigen-positive cells in mosquito and neural cell lines. DENV-3 antigen-positive cells are shown as determined by using immunofluorescence (IFA) staining at 48 hours postinfection at a multiplicity of infection (MOI) of 0.01. DENV-3 isolated from the cerebrospinal fluid (CSF) specimen (CSF-11098) and the serum specimen (HP-5528) were separately inoculated to C6/36, SKNSH, T98G, and N2A cell lines, and viral antigen (E NS1, NS2B, NS3, NS4A, NS4B, and NS5) were detected, respectively, by using IFA. Infected cells (%) were determined by average values (infected/total infected and noninfected cells) × 100% in three random fields at a magnification 200x. P value was determined by the t-test. P value 0.05 was considered significant.

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