Tracing the Negative Results of Multiplex Real-Time PCR Assay for Diagnosis of Bacterial Pediatrics Meningitis

Table 1

Primer sequencing in multiplex real-time PCR.

Bacteria	Gene	Primer sequencing (5′ to 3′)	Nucleotides target	Reference
N. meningitidis	CTR-F	GCT​GCG​GTA​GGT​GGT​TCA​A	617–635	[8]
	CTR-R	TTG​TCG​CGG​ATT​TGC​AAC​TA	727–708
Hib	bexA-F	GGC​GAA​ATG​GTG​CTG​GTA​A	142–160	[8]
	bexA-R	GGC​CAA​GAG​ATA​CTC​ATA​GAA​CGT​T	241–217
S. pneumoniae	Ply-F	TGC​AGA​GCG​TCC​TTT​GGT​CTA​T	849–915	[8]
	Ply-R	CTC​TTA​CTC​GTG​GTT​TCC​AAC​TTG​A	974–950