Tracing the Negative Results of Multiplex Real-Time PCR Assay for Diagnosis of Bacterial Pediatrics Meningitis

Table 2

Primer sequencing of specific real-time PCR.

Bacteria	Primers	Primers and probe sequence	Explanations	References
N. meningitidis	sodC-F	GCA​CAC​TTA​GGT​GAT​TTA​CCT​GCA​T	—	[9]
	sodC-R	CCA​CCC​GTG​TGG​ATC​ATA​ATA​GA
	sodC-P	CAT​GAT​GGC​ACA​GCA​ACA​AAT​CCT​GTT​T	5′ FAM, 3′ BHQ1
H. influenzae	Hpd-F	GGT​TAA​ATA​TGC​CGA​TGG​TGT​TG	—	[9]
	Hpd-R	TGC​ATC​TTT​ACG​CAC​GGT​GTA
	Hpd-P	TTG​TGT​ACA​CTC​CGT“T”GGT​AAA​AGA​ACT​TGC​AC	5′ FAM, BHQ1 on “T,” 3′ SpC6
S. pneumoniae	lytA-F	ACG​CAA​TCT​AGC​AGA​TGA​AGC​A	—	[9]
	lytA-R	TCG​TGC​GTT​TTA​ATT​CCA​GCT
	lytA-P	TGC​CGA​AAA​CGC“T”TGA​TAC​AGG​GAG	5′ FAM, BHQ1 on “T,” 3′ SpC6