Establishment and Performance Evaluation of Multiplex PCR-Dipstick DNA Chromatography for <i>Mycoplasma pneumoniae</i> and <i>Chlamydia pneumoniae</i> Rapid Detection

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr class="thead"><td class="align_left">Primers</td><td class="align_center">Gene</td><td class="align_center">Tag</td><td class="align_center">Sequence (5′-3′)</td><td class="align_center">Amplicon size (bp)</td></tr><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr><td class="align_left">MP-F</td><td class="align_center" rowspan="2">RI3</td><td class="align_center" rowspan="2">tag​cta​agt​ggt​cca​taa​ct</td><td class="align_center">Tag-C3-AAG​GAA​GCT​GAC​TCC​GAC​AA</td><td class="align_center" rowspan="2">107</td></tr><tr><td class="align_left">MP-R</td><td class="align_center">Biotin-CCAAACACCAAAAGCGCTTG</td></tr><tr><td class="align_left" colspan="5"><hr/></td></tr><tr><td class="align_left">CP-F</td><td class="align_center" rowspan="2">AR39</td><td class="align_center" rowspan="2">ctg​cgg​gta​tag​aag​ccc​t</td><td class="align_center">Tag-C3-GCA​CTT​TCA​TGG​GAG​CCT​TT</td><td class="align_center" rowspan="2">92</td></tr><tr><td class="align_left">CP-R</td><td class="align_center">Biotin-GCGCATTCCAAAAGCTTCAC</td></tr><tr><td class="align_left" colspan="5"><hr/></td></tr><tr><td class="align_left">Internal control: F</td><td class="align_center" rowspan="2">NM-005157</td><td class="align_center" rowspan="2">gct​cta​gcc​acc​aat​gaa​tct​aa</td><td class="align_center">Tag IC-C3-CAGAGCACAGAGACACCACT</td><td class="align_center" rowspan="2">153</td></tr><tr><td class="align_left">Internal control: R</td><td class="align_center">Biotin-GGCGCTCATCTTCATTCAGG</td></tr><tr class="table-tr"><td colspan="5"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td><div>F: forward primer; R: reverse primer.<br/></div></td></tr></table>

<div>The primers for the PCR-dipstick DNA chromatography assay.</div>

Canadian Journal of Infectious Diseases and Medical Microbiology

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Table 1

Table 1: Establishment and Performance Evaluation of Multiplex PCR-Dipstick DNA Chromatography for <i>Mycoplasma pneumoniae</i> and <i>Chlamydia pneumoniae</i> Rapid Detection 

Table 1 | Establishment and Performance Evaluation of Multiplex PCR-Dipstick DNA Chromatography for Mycoplasma pneumoniae and Chlamydia pneumoniae Rapid Detection 