First Evidence of Fowl Adenovirus Induced Inclusion Body Hepatitis in Chicken in Bangladesh

Table 1

Clinical signs, postmortem lesions and molecular detection of fowl adenoviruses in tissue samples (n = 50) obtained from ten different flocks of chicken.

Flock type	Flock size	Location	Age (days)	Sampling time	Morbidity/Mortality (%)	Clinical signs observed	Postmortem lesions	FAdVwith serotype	GenBank accession number
Broiler (B1)	2,000	Gazipur Sadar	9	June 2021	25/15	Depression, Reluctant to move	Enlarged hemorrhagic liver, swollen kidneys	5/5; S-8b	OP004931
Broiler (B2)	2,000	Gazipur Sadar	7	June 2021	23/20	Drowsiness, ruffled feathers	Tan colored liver, swollen spleen & kidneys	5/5; S-8b	OP502745
Broiler (B3)	2,000	Gazipur Sadar	11	June 2021	30/16	Drowsiness, less body weight gain, respiratory distress	Hemorrhagic liver, hydrocardium, swollen kidneys	5/5; S-8b	—
Broiler breeder (BB1)	10,000	Gazipur Sadar	14	December 2021	35/25	Drowsiness, lack of uniformity	Enlarged liver with hemorrhage, swollen kidneys & spleen	2/5; S-5	OP454906
Broiler breeder (BB2)	10,000	Gazipur Sadar	9	December 2021	40/20	Drowsiness, ruffled feathers reluctant to move	Hemorrhagic enlarged liver, congested pancreas, kidneys	5/5; S-5; 3/5; S-8b	S-5; OP502746; S-8b-; OP502747
Broiler breeder (BB3)	10,000	Atwary, Panchagarh	8	June 2022	45/20	Drowsiness, Respiratory distress	Enlarged liver, fluid in pericardial sac, swollen kidneys with hemorrhage	5/5; S-8b	OP454908
Broiler breeder (BB4)	10,000	Atwary, Panchagarh	14	June 2022	40/15	Drowsiness, depression, respiratory distress	Hemorrhagic enlarged liver & hemorrhage at muscle & kidneys	5/5; S-8b	—
Broiler breeder (BB5)	10,000	Tetulia, Panchagarh	16	February 2021	35/12	Drowsiness, ruffled feather, sudden death	Pale enlarged liver, swollen kidneys	2/5; S-11	—
Broiler breeder (BB6)	10,000	Tetulia, Panchagarh	15	February 2021	65/30	Drowsiness, Anorexia, unable to move	Pale enlarged liver, swollen kidneys	3/5; S-11	OP454907
Commercial layer (CL1)	5,000	Dinajpur Sadar	35	November 2020	10/5	Anorexia, Reluctant to move	Enlarged liver	0/5

Number of sample positive/number of sample tested. S-8b: serotype 8b, S-5: serotype 5, S-11: Serotype 11; —: sequencing not done but serotype was detected with serotype specific realtime PCR kit as mentioned in the methodology section.