A Unique Combination of Mn²⁺ and Aluminum Adjuvant Acted the Synergistic Effect

Figure 3

Proliferative responses of spleen lymphocyte and bacterial load in organs from mice. (a) Proliferative response of splenocytes was assessed with a lymphocyte proliferation assay. Seven days after the last immunization, the spleen lymphocytes from the immunized mice (n = 3) were obtained, and these cells were treated with IsdB3 proteins for 48 h. A significant difference was observed in the IsdB3 + Mn²⁺ + AH group, in comparison with the IsdB3 group, IsdB3 + Mn²⁺ group, IsdB3 + AH group, and PBS group. (b–d) Bacterial load in organs was analyzed fourteen days after the last immunization. The immunized mice (n = 3) were intraperitoneally treated with the dose of 3 × 10⁸ CFU of S. aureus strain Newman, and after 48 h, these mice were sacrificed, and their livers (b), spleens (c), and kidneys (d) were obtained, and the grinding fluid of these organs was used to detect the CFU of bacterial colonization. The data were shown as the mean ± SD (n = 3). Significant differences were indicated by , , and .

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