Pasteurella multocida Toxin Aggravates Ligatured-Induced Periodontal Bone Loss and Inflammation via NOD-Like Receptor Protein 3 Inflammasome

Figure 5

MCC950 ameliorates mice periodontitis, while PMT aggravates mice periodontitis. (a) Reconstructed 3D micro-CT images of the control, lig+NS, lig+MCC950, and lig+PMT groups. (b) BMD, BV, and BV/TV analysis of control, lig+NS, lig+MCC950, and lig+PMT groups. (c) H&E staining and the distances between the CEJ and the ABC from mesial side of f control, lig+NS, lig+MCC950, and lig+PMT groups. (d) Immunohistochemistry staining of IL-1β in the gingivae of the control, lig+NS, lig+MCC950, and lig+PMT groups. The histogram shows the quantification of average optical density (integrated optical density (IOD)/area) of IL-1β immunohistochemistry staining (/group). Values are presented as (, , and ). Abbreviations: ABC=alveolar bone crest; BMD=bone mineral density; BV=bone volume; BV/TV=bone volume/tissue volume ratio; CEJ=cemento-enamel junction; H&E = hematoxylin and eosin; IL=interleukin; micro-CT=microcomputed tomography; NS=normal saline; PMT=Pasteurella multocida toxin; 3D=three-dimensional.

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