miR-30c Increases the Intracellular Survival of Helicobacter pylori by Inhibiting Autophagy

Figure 1

H. pylori infection induced incomplete autophagy flux in GES-1 cell lines. (a) GES-1 cells were infected with H. pylori () for different times. H. pylori infection increases the expression of LC3B-II in time-dependent manner (). But no obvious alteration of P62 protein level was observed (). (b) The GES-1 cells were pretreated with Baf A 1 (10 nM) and then stimulated with H. pylori () (). (c) After GES-1 cells were transfected with mRFP-GFP-LC3 adenovirus for 24 h, the cells were stimulated with H. pylori for another 12 h. The number of puncta in each cell was counted. H. pylori-infected GES-1 cells can significantly increase the number of autophagosomes and autolysosomes (). All error bars indicate SEM. , , and .

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