miR-30c Increases the Intracellular Survival of Helicobacter pylori by Inhibiting Autophagy

Figure 4

miR-30c-1-3p and miR-30c-5p regulated target autophagy-related genes at mRNA level through mRNA degradation. (a) Underlying target site for miR-30c-1-3p on the 3UTR of ATG14 and ULK1 mRNA and underlying target site for miR-30c-5p on the 3UTR of ATG12 mRNA were forecast by TargetScan7.2 and miRDB. (b) Dual-luciferase reporter assay was used to confirm the interactions between miR-30c-1-3p and 3UTR of ATG14 and ULK1, as well as miR-30c-5p and 3UTR of ATG12. (c) GES-1 cells were transfected with mimic (100 nM) and inhibitor (100 nM) of miR-30c-1-3p as well as miR-30c-5p, negative mimic (100 nM) or negative inhibitor (100 nM) for 36 h. The mRNA levels of ATG14, ULK1 and ATG12 were detected by RT-qPCR (). All error bars indicate SEM. , , and .

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