Severe Inflammation Caused by Coinfection of PCV2 and Glaesserella parasuis Is Associated with Pyroptosis via Noncanonical Inflammasome Pathway

Figure 3

Phagocytosis and clearance of G. parasuis by RAW 264.7 cells to coinfection with PCV2. (a) RAW264.7 cells were initially infected with PCV2 () for 24 h and then infected with G. parasuis () for 1 h, and the medium was replaced with fresh complement DMEM containing penicillin and gentamicin for 1 h, and then, the cells were washed, lysed, and streaked onto TSA for CFU count. (b) The treated RAW264.7 cells were lysed and streaked onto TSA for CFU count at 2 h, 4 h, 6 h, and 8 h, respectively.

(a)

(b)