Plasmodium berghei-Mediated NRF2 Activation in Infected Hepatocytes Enhances Parasite Survival

Figure 5

Parasite survival is reduced in NRF2-deficient cells. (a) HuH7 WT and 4 different NRF2^-/- clones were infected with PbmCherry. At 6 and 48 hpi, parasite numbers were evaluated using automated high throughput live cell imaging and analysis (InCell Analyzer 2000). The graph shows relative parasite survival from 6 to 48 hpi compared to the WT control. Mean and SD of three independent experiments are depicted. parasites per cell line and experiment. A one-way ANOVA test coupled to a Tukey’s post hoc test was used to determine -values (). (b) Parasite size at 48 hpi of experiment described in (a). The graph shows the medians in parasite size of >200 parasites per cell line and experiment. The experiment was performed 3 times. Mean and SD are depicted. parasites per cell line and experiment. -values were calculated using a one-way ANOVA test followed by Tukey’s post hoc test (ns: ). (c) HuH7 WT and 2 different p62^-/- clones with the corresponding addback cell lines were infected with P. berghei parasites expressing GFP (PbGFP). Parasite survival in the different cell lines was determined as in (a). The graph shows the relative survival compared to the WT control. Mean and SD of three independent experiments are shown. parasites per cell line and experiment. -values were calculated using a one-way ANOVA test coupled to Tukey’s post hoc test (ns: ; ; and ). (d) Parasite size at 48 hpi of the experiment described in (c). The graph shows the medians in parasite size of 4 experiments. parasites per cell line and experiment. Mean and SD are depicted. -values were determined using a one-way ANOVA test followed by Tukey’s post hoc test (ns: p >0.05; ; and ).

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