Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells

Figure 1

Detection of intracellular and extracellular ROS in T84 and Caco-2 cells after infection with C. jejuni 11168H, 81-176, or 488 strains. Intracellular ROS in T84 cells after infection with C. jejuni for (a) 3 hours or (b) 24 hours and extracellular ROS from T84 cells after infection with C. jejuni for (c) 3 hours or (d) 24 hours were measured. Intracellular ROS in Caco-2 cells after infection of C. jejuni for (e) 3 hours or (f) 24 hours and extracellular ROS from Caco-2 cells after infection for (g) 3 hours or (h) 24 hours were measured. For detection of intracellular ROS, DCFDA was used. For detection of extracellular ROS, Amplex® Red reagent with HRP was used. H₂O₂ was used as a positive control. Experiments were repeated in three biological and three technical replicates. Asterisks denote a statistically significant difference (; ; ).

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